(a) 15.8% of a 340 nm light is trasmitted through a cell of 1 cm. The cell contains a liquid solution of NADH. The molar absorptivity constant () of NADH for this wave length is = 6.22 106 cm2/mol. What is the concentration of NADH in the solution?

(b) The absorbance of a solution of an enzyme and AMP (Adenosine monophosphate) in a cell of 1 cm is found to be 0.46 for light with = 280 nm and 0.58 for light with = 260 nm. What are the concentrations of the enzyme and AMP ? (Hint: you have two equations and two unknown variables) The molar absorptivity constants for the two species are given in the following table:

280 cm /mol 260 [cm/mol Enzyme (E) 2.96 - 107 1.52 - 107 AMP 2.4 - 106 1.50 - 107 (a) 15.8% of a 340 nm light is trasmitted through a cell of 1 cm. The cell contains a liquid solution of NADII. The molar absorptivity constant (e) of NADII for this wave length is = 6.22 - 106 cm/mol. What is the concentration of NADII in the solution? (b) The absorbance of a solution of an enzyme and AMP (Adenosine monophosphate) in a cell of 1 cm is found to be 0.46 for light with = 280 nm and 0.58 for light with i = 260 nm. What are the concentrations of the enzyme and AMP ? (Ilint: you have two equations and two unknown variables) The molar absorptivity constants for the two species are given in the following table: 280 (cm/mol 260 (cm/mol Enzyme (E) 2.96 - 107 1.52 - 107 AMP 2.4 - 106 1.50 -107