A 2 year old boy is diagnosed with a pyruvate carboxylase deficiency. DNA sequencing from a sample from the patient reveals the existence of a mutation in the gene encoding the enzyme pyruvate carboxylase. The physician then collaborates with the biochemist in order to observe the effects of the mutation on the function of the Pyruvate carboxylase by using both normal pyruvate carboxylase and mutant pyruvate carboxylase carrying the mutation identified in the boy's DNA. The graph in the figure below summarizes the data obtained by the biochemist. Which statement would best describe the mutation in this patient? - The mutation is most likely found away from the active site, affecting the binding of the substrate to the enzyme. - The mutation is most likely found away from the active site, affecting the amount of product formed. - The mutation is most likely found on the active site and affects both the binding of the substrate and the amount of product formed. - The mutation is most likely found in the active site, affecting the binding of the substrate to the enzyme. - The mutation is most likely found in the active site, affecting the amount of product formed