A 500. L aliquot of a caffeine unknown and the internal standard (theophylline) is diluted to a

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A 500. μL aliquot of a caffeine unknown and the internal standard (theophylline) is diluted to a total volume of 10.00 mL. A calibration curve for caffeine was generated using internal standards on the HPLC and gave the equation of the curve: Caffeine/Theophylline Area Ratio = 1.181 × Caffeine Concentration (ppm) + 0.027 If the HPLC peak for the diluted unknown has an Caffeine/Theophylline area ratio is 3.86 , what is the original caffeine concentration in ppm? Report your answer to 2 decimal places. Hint: Remember the dilution factor A 500. μL aliquot of a caffeine unknown and the internal standard (theophylline) is diluted to a total volume of 10.00 mL. A calibration curve for caffeine was generated using internal standards on the HPLC and gave the equation of the curve: Caffeine/Theophylline Area Ratio = 1.181 × Caffeine Concentration (ppm) + 0.027 If the HPLC peak for the diluted unknown has an Caffeine/Theophylline area ratio is 3.86 , what is the original caffeine concentration in ppm? Report your answer to 2 decimal places. Hint: Remember the dilution factor