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A bioaerosol was sampled at the air flowrate of 4 liters per minute over 15 minutes sampling period. The sampling device was filled with 50mL

A bioaerosol was sampled at the air flowrate of 4 liters per minute over 15 minutes sampling period. The sampling device was filled with 50mL of collecting liquid and all collected microorganisms remained in it over the entire duration of the procedure with no losses due to re-aerosolisation of the liquid. On completion of sampling, 0.1mL aliquot of collecting liquid was spread onto the surface of the agar plate and incubated over night. Then the colonies on the agar plate were counted and the result was 30 Colony Forming Units (CFU). What was the initial concentration of biological aerosol in the air in CFU/m3?


a. 450000 CFU/m3

b. 350000 CFU/m3

c. 250000 CFU/m3

d. 150000 CFU/m3


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