a. Calculate the total serum calcium concentration using the mean SCa result (allowing for dilutions) as follows:

b. Calculate the total serum magnesium concentration using the mean SMg result. [Be sure to take into account the dilution considerations.]

c. Calculate the ultrafiltrate serum calcium concentration using the mean of the non-rejected UCa data. [Be sure to take into account the dilution considerations.]

d. Calculate the ultrafiltrate serum magnesium concentration using the mean of the non-rejected UMg values. [Remember to consider dilution issues.]

e. Calculate the percentage (%) of total serum calcium and magnesium that are in ultrafiltrate form.

Results: - In section C we used 30uL (not 300uL) - In section G for 1% Nitric Acid we used 9.97mL (not 9.7mL ) This was because the horse serum was too concentrated for the standards to reach an agreed calibration curve on which to base The Spectro results are as follows: SPr1=0.377 SPr2=0.291 SPr3=0.244 SPr4=0.240 UPr1=0.001 UPr-2 =0.002 Pr3=0.002 UPr4=0.005 lg(285.213nm) itensity =566352.373 Concentration 22.795 orrelation coefficient: 0.99999 Ca(393.366nm) Intensity =2744857.404 * Concentration 1.130 Correlation coefficient: 0.99966 Results: - In section C we used 30uL (not 300uL) - In section G for 1% Nitric Acid we used 9.97mL (not 9.7mL ) This was because the horse serum was too concentrated for the standards to reach an agreed calibration curve on which to base The Spectro results are as follows: SPr1=0.377 SPr2=0.291 SPr3=0.244 SPr4=0.240 UPr1=0.001 UPr-2 =0.002 Pr3=0.002 UPr4=0.005 lg(285.213nm) itensity =566352.373 Concentration 22.795 orrelation coefficient: 0.99999 Ca(393.366nm) Intensity =2744857.404 * Concentration 1.130 Correlation coefficient: 0.99966