A carbohydrate (S) decomposes in the presence of an enzyme (E). The Michaelis-Menten kinetic parameters were found to be as follows: = / = /

a. Calculate the change of substrate concentration with time in a batch reactor. The initial substrate concentration is 400 mol/m3 . Have the time go from 0 minutes to 60 minutes with increments of 1 minutes to create the plot.

b. Assume that you obtained experimentally the CS versus t curve you calculated in part (a). Estimate KM and VMAX by plotting the (CS0 CS)/ln(CS0/CS) versus t/ln(CS0/CS) curve, according to the equation found in Lecture 10. Is this approach reliable?

Use the following equation to compute KM and Vmam

Form 2: Combine k2 and CE,TOT into the slope (when performing experiments at various CE, )