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A culture of bacteria that secrete a product in a chemostat operated at steady state. The specific growth rate of biomass is adequately described by
A culture of bacteria that secrete a product in a chemostat operated at steady state. The specific growth rate of biomass is adequately described by the Monod equation, and the volumetric rate of product formation is described by LeudekingPiret kinetics: dPdt alpha mu beta Chi
This system is well characterized, such that the following constants are known:
Yxs gXgS
alpha gPgX
S gSL
max h
beta gPgXh
Ks gL
Yps gPgS
V L
The liquid feed to the chemostat is sterile and the flow rates entering and exiting the chemostat are equal.
a What should the flow rate be adjusted to in this chemostat to achieve the optimal productivity of product formation?
b What is the specific rate of product formation during cell maintenance alone?
c Define the a coefficient in LeudekingPiret kinetics. What is the total specific rate of product formation?
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