Question
a. Derivatization is the process of chemically modifying a compound to produce a new compound which has properties that are suitable for analysis. i.
a. Derivatization is the process of chemically modifying a compound to produce a new compound which has properties that are suitable for analysis. i. Give four reasons why derivatization is necessary in gas chromatography. (2 marks) ii. With help of equations, explain the following derivatization techniques. A. Silylation. B. Acylation (2% marks) (2% marks) b. The Figure below is diagram for electron capture detector (ECD), study it and answer the question below. Collector Outlet Anode (4) Cathode (-) Radioactive Column Effluent surface i. Explain basis of its operations. (4 marks) ii. Name four species that are separated by this detector. iii. Name two advantages of using this detector. (2 marks) (1 mark) QUESTION SIX (14 MARKS) a. There are four terms in Van Deemeter equation namely, mutiple path, longitudinal diffusion, mass transfer and flow rate which contribute to the band broadening. Explain how the following terms contribute to band broadening. i. Mutiple path. ii. Longitudianal diffussion. iii. Mass transfer. (3 marks) b. Sketch a well label diagram for an HPLC system that is capaple of an isocratic elution. Specify detector that is ideal for this system. (4 marks)
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