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A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290kDa with elution volume 20.8mL serum albumin,

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A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290kDa with elution volume 20.8mL serum albumin, 37kDa with elution volume 42.5mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume. The elution volume of an unknown protein was 28.5. Use the equation just determined above to estimate the molar mass of the protein in kDa units. Please don't round your answer until the end to ensure your answer is within the accepted range. Round your answer to the nearest whole number (e.g. If you calculated 27.52, input vour answer as 28)

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