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A laser light with an intensity of 1 mW at 500 nm is illuminating water to measure the Raman spectrum of water. What is
A laser light with an intensity of 1 mW at 500 nm is illuminating water to measure the Raman spectrum of water. What is the ratio of Rayleigh scattered light intensity to Raman scattered light intensity if we are detecting with a lens/detector combination at a 90 angle. We are adding a fluorophore with a absorption cross-section of 4 x 10-19 cm at 500 nm and a Quantum efficiency of 0.5 into the mixture. What is the concentration of the fluorophore that will produce the same intensity as the Raman scattering of the water? Why is this number important? How can you detect the Raman spectrum of water at the presence of high concentrations of this fluorophore. Hint: The number density for water is 3.3x10 cm-3
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