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A new preparation of -galactosidase (lactase) is being advertised for analysis work at a cost that is 5% cheaper than the existing preparation. Due to
A new preparation of -galactosidase (lactase) is being advertised for analysis work at a cost that is 5% cheaper than the existing preparation. Due to the decrease in cost, there is some doubt as to whether the new preparation is as pure as the existing -galactosidase preparation used by your company. Before placing a new bulk order for the new -galactosidase preparation, your company has obtained a test sample of the new preparation and this has been tested as a free enzyme in solution in an experimental stirred tank bioreactor, and compared with the existing -galactosidase preparation. The results o these experiments, at pH=7 and 30C are as follows: Enzyme preparation B: New enzyme. [E]0= 40.0ugt1 \begin{tabular}{|c|c|} \hline Initiallactosesugarsubstrateconcentration[S](mgl1) & Initialreactionrater(mgl1s1) \\ \hline 2.0000 & 0.2500 \\ \hline 1.0000 & 0.2000 \\ \hline 0.4000 & 0.1250 \\ \hline 0.2000 & 0.0769 \\ \hline \end{tabular} Using the data provided in Question 1, determine whether there is any inhibition of the new cheaper preparation. If so, identify the type of inhibition. Yes, the new preparation enzyme is inhibited, and the inhibition is competitive. Yes, the new preparation enzyme is inhibited, and the inhibition is uncompetitive. Yes, the new preparation enzyme is inhibited, and the inhibition is non-competitive. There is no inhibition in the new preparation enzyme. none of the above
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