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A newly discovered enzyme, MBGase, cleaves carbon - carbon bonds of vicinal alcohols. The kinetic parameters were measured for 1 . 0 nM of MBGase

A newly discovered enzyme, MBGase, cleaves carbon-carbon bonds of vicinal alcohols.The kinetic parameters were measured for 1.0 nM of MBGase and determined to be a Km =10 mMand a kcat =100 s-1
(a) Calculate Vmax for the enzyme.
(b) Calculate the initial velocity, Vo, if the vicinal alcohol concentration is 90 nM.
(c) Sketch the Michaelis-Menten plot and label:
-the axes (with units)
-the point on the curve at which the vicinal alcohol concentration is at 10 nM
-Vmax
(d) Sketch the graph in part (c) as a double-reciprocal plot (Lineweaver-Burke Plot) and label:
-the axes (with units)
-the point on the curve that can be used to determine Km
-the point on the curve that can be used to determine Vmax

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