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A newly discovered enzyme, MBGase, cleaves carbon - carbon bonds of vicinal alcohols. The kinetic parameters were measured for 1 . 0 nM of MBGase
A newly discovered enzyme, MBGase, cleaves carboncarbon bonds of vicinal alcohols.The kinetic parameters were measured for nM of MBGase and determined to be a Km mMand a kcat s
a Calculate Vmax for the enzyme.
b Calculate the initial velocity, Vo if the vicinal alcohol concentration is nM
c Sketch the MichaelisMenten plot and label:
the axes with units
the point on the curve at which the vicinal alcohol concentration is at nM
Vmax
d Sketch the graph in part c as a doublereciprocal plot LineweaverBurke Plot and label:
the axes with units
the point on the curve that can be used to determine Km
the point on the curve that can be used to determine Vmax
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