Question
A purified protein has a molecular mass of 360 kDa when measured by size exclusion chromatography. When analyzed by gelelectrophoresis in the presence of SDS,
A purified protein has a molecular mass of 360 kDa when measured by size exclusion chromatography. When analyzed by gelelectrophoresis in the presence of SDS, three bands are observed, with molecular masses of 160, 140, and 60 kDa. When gelelectrophoresis is carried out in the presence of SDS anddithiothreitol, three bands are once again observed, with molecular masses of 140, 80, and 60 kDa. What is the subunit composition of the protein?
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From the data given it is clear that the protein has four subunits with molecular masses of ...
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Biochemistry Concepts and Connections
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
1st edition
321839927, 978-0133853490, 133853497, 978-0321839923
