A scientist wants to determine whether bacterial species with different lifestyles present different genome sizes. Load the tableYourdata into R (you have previously used this dataset). As a reminder, the third column of the table indicates the genome size of each species (in Mb) and the fourth column indicates the lifestyle of each species.

1)Let's assume that the genome sizes of the dataset arenormally distributed and that thevariance is the same across lifestyles. Use a test in R to determine whether bacterial species with different lifestyles present similar of different genome sizes (with alpha=0.05).

--use aov()

2) From the result of the previous test, identifies which lifestyles present the most significantly different genome sizes.

--useTukeyHSD()

3)Let's assume now that the genome sizes of the dataset arenot normally distributed or that the variance isdifferent across lifestyles. Use a test in R to determine whether bacterial species with different lifestyles present similar of different genome sizes (with alpha=0.05).

--use Kruskal Wellis test

Acinetobacter_baumannii 363 4.01980082902 free_living positive Acinetobacter_pittii 34 4.0212 free_living positive Actinobacillus_pleuropneumoniae 15 2.27236913043 animal_pathogen negative Aeromonas_hydrophila 29 4.85646560976 free_living negative Aggregatibacter_actinomycetemcomitans 20 2.18749576923 commensal negative Bacillus_amyloliquefaciens 31 4.08667916667 free_living negative Bacillus_anthracis 21 5.42669035398 animal_pathogen positive Bacillus_cereus 169 5.75466234568 free_living negative Bacillus_coagulans 15 3.296639 free_living positive Bacillus_licheniformis 15 4.29817136364 free_living negative Bacillus_methylotrophicus 24 3.99684233333 free_living negative Bacillus_pumilus 26 3.72804774194 free_living negative Bacillus_subtilis 54 4.12654443182 free_living negative Bacillus_thuringiensis 24 6.11565164384 animal_pathogen negative Bacteroides_fragilis 66 5.45355980769 commensal positive Bifidobacterium_bifidum 19 2.20993428571 commensal positive Bifidobacterium_breve 26 2.30116242424 commensal negative Bifidobacterium_longum 40 2.4646825 commensal negative Bordetella_bronchiseptica 40 5.24891484848 animal_pathogen positive Bordetella_pertussis 32 3.69379097015 animal_pathogen negative Borrelia_burgdorferi 14 1.1859964878 animal_pathogen positive Brachyspira_hyodysenteriae 19 3.05181095238 animal_pathogen negative Bradyrhizobium_japonicum 17 9.15084047619 plant_commensal positive Brucella_abortus 39 3.29129508982 animal_pathogen positive Brucella_melitensis 25 3.29230625 animal_pathogen negative Brucella_suis 21 3.32083436364 animal_pathogen negative Burkholderia_cenocepacia 21 7.69713258065 free_living negative Burkholderia_cepacia 53 8.33828011111 free_living negative Burkholderia_multivorans 27 6.90025378378 free_living negative Burkholderia_pseudomallei 174 7.14722332487 free_living negative Burkholderia_stagnalis 24 7.64343111111 free_living negative Burkholderia_territorii 25 6.89213580645 free_living positive Burkholderia_ubonensis 188 7.52502743083 free_living negative Burkholderia_vietnamiensis 29 6.86852583333 free_living negative Campylobacter_fetus 22 1.86594935484 animal_pathogen positive Campylobacter_jejuni 345 1.71711073906 animal_pathogen positive Chlamydia_psittaci 17 1.19581388889 intracellular_pathogen positive Chlamydia_trachomatis 68 1.44271992188 intracellular_pathogen positive Citrobacter_freundii 22 5.27213471698 free_living positive Clostridium_botulinum 74 3.80489368098 free_living positive Clostridium_perfringens 15 3.52985666667 free_living negative Comamonas_testosteroni 20 5.573342 free_living positive Corynebacterium_diphtheriae 18 2.43461958333 animal_pathogen negative Corynebacterium_pseudotuberculosis 26 2.34107243243 animal_pathogen negative Dehalococcoides_mccartyi 15 1.421365625 free_living positive Enterobacter_aerogenes 67 5.23946760417 commensal negative Enterobacter_asburiae 35 4.94198042553 commensal positive Enterobacter_cloacae 221 4.99472384615 commensal positive Enterobacter_hormaechei 39 5.09606885246 commensal positive Enterococcus_faecalis 179 3.05658051919 commensal positive Enterococcus_faecium 181 2.90917669048 commensal positive Escherichia_albertii 31 4.7702565625 commensal negative Escherichia_coli 400 5.13930008292 commensal positive Fusobacterium_nucleatum 30 2.33107682927 commensal negative Gallibacterium_anatis 21 2.492255 commensal negative Gardnerella_vaginalis 25 1.61314978261 animal_pathogen negative Haemophilus_influenzae 89 1.8705657377 animal_pathogen negative Haemophilus_parainfluenzae 15 2.02616235294 commensal positive Helicobacter_pylori 303 1.6306014902 animal_pathogen positive Kingella_kingae 41 2.00099304348 commensal positive Klebsiella_oxytoca 52 6.13582649351 free_living positive Klebsiella_pneumoniae 295 5.63934828326 free_living positive Lactobacillus_casei 24 3.01169147059 commensal positive Lactobacillus_crispatus 20 2.2070868 commensal negative Lactobacillus_delbrueckii 19 1.88856413793 commensal negative Lactobacillus_fermentum 15 2.02699105263 commensal negative Lactobacillus_helveticus 15 2.04424227273 commensal positive Lactobacillus_kunkeei 15 1.54320176471 commensal negative Lactobacillus_paracasei 36 2.94431132075 commensal positive Lactobacillus_plantarum 43 3.30323107143 commensal negative Lactobacillus_rhamnosus 30 2.97860532258 commensal positive Lactococcus_lactis 55 2.52310657143 free_living positive Legionella_pneumophila 111 3.46702419355 free_living positive Leptospira_borgpetersenii 15 3.89372032258 animal_pathogen positive Leptospira_interrogans 113 4.67965864769 animal_pathogen negative Leptospira_kirschneri 20 4.43203037037 animal_pathogen positive Leptospira_noguchii 15 4.71334533333 animal_pathogen negative Leptospira_santarosai 30 4.03320657143 animal_pathogen positive Leptospira_weilii 14 4.25398588235 animal_pathogen positive Leuconostoc_mesenteroides 15 1.915335625 free_living negative Listeria_monocytogenes 110 2.98469117371 free_living negative Methanosarcina_mazei 25 4.070018125 free_living positive Microcystis_aeruginosa 16 4.97376647059 free_living positive Moraxella_catarrhalis 19 1.90384043478 commensal negative Morganella_morganii 15 3.98036294118 commensal positive Mycobacterium_abscessus 42 5.14822948413 free_living negative Mycobacterium_africanum 20 4.39781666667 animal_pathogen positive Mycobacterium_bovis 17 4.30371632353 animal_pathogen positive Mycobacterium_tuberculosis 400 4.37934894693 animal_pathogen positive Mycoplasma_pneumoniae 22 0.815550444444 intracellular_pathogen negative Neisseria_gonorrhoeae 93 2.1358724159 animal_pathogen positive Neisseria_meningitidis 255 2.15741877883 animal_pathogen positive Oenococcus_oeni 36 1.79989532258 free_living positive Paenibacillus_polymyxa 19 5.8272605 free_living positive Pasteurella_multocida 27 2.27754581395 commensal negative Pectobacterium_carotovorum 36 4.79050625 plant_pathogen positive Peptoclostridium_difficile 76 4.11957380952 free_living negative Porphyromonas_gingivalis 17 2.32024894737 animal_pathogen positive Prochlorococcus_marinus 24 1.76564322581 free_living negative Propionibacterium_freudenreichii 19 2.5970447619 free_living positive Proteus_mirabilis 32 3.961596 commensal positive Pseudomonas_aeruginosa 400 6.65286135614 free_living negative Pseudomonas_amygdali 27 6.1258234 free_living positive Pseudomonas_chlororaphis 15 6.815334375 free_living negative Pseudomonas_denitrificans 14 6.62127764706 free_living positive Pseudomonas_fluorescens 78 6.23487766667 free_living negative Pseudomonas_putida 42 6.03494732143 free_living negative Pseudomonas_stutzeri 27 4.64394111111 free_living positive Pseudomonas_syringae 115 6.0698992973 plant_pathogen negative Salinispora_arenicola 38 5.68606510638 free_living positive Salinispora_pacifica 32 5.32121275 free_living negative Salmonella_enterica 400 4.81276317931 commensal positive Serratia_marcescens 100 5.24200341297 free_living positive Staphylococcus_aureus 400 2.86586926357 commensal negative Staphylococcus_epidermidis 124 2.56697687296 commensal negative Staphylococcus_haemolyticus 88 2.5780619209 commensal positive Staphylococcus_warneri 15 2.5123775 commensal negative Stenotrophomonas_maltophilia 85 4.73005565217 free_living positive Streptococcus_agalactiae 177 2.08594817473 commensal positive Streptococcus_anginosus 18 1.9695045 commensal positive Streptococcus_dysgalactiae 13 2.087086 commensal negative Streptococcus_equi 21 2.14537844898 animal_pathogen negative Streptococcus_gordonii 18 2.18908363636 commensal negative Streptococcus_mitis 45 1.98134846154 commensal negative