A single stage continuous culture of Corynebacterium glutamicum bacteria is conducted in a 200L stirred fermenter, at 30C. The reactor is sparged with a gas mixture of 63% oxygen and 37% nitrogen at a rate of 1 VVM (Volume/Volume.min) at room temperature. Average pressure in the fermenter is 1atm. Volumetric flowrate of off-gas from the fermenter is 189L min1. Exit gas stream from the fermenter has been analysed and found that it contains 52% O2 1. What is the oxygen transfer rate? 2. If the maximum specific oxygen uptake rate of the microorganism is 5.103 g/(L.sec), is the cell growth in the fermenter limited by mass transfer? Explain shortly. 3. What will be the value of volumetric mass transfer coefficient, kLa, in the reactor assuming that the saturated concentration of dissolved oxygen in equilibrium with air is 7.8mg/L. The culture maintains a dissolved oxygen concentration which is 38% of the saturated value. 4. What will be the cell productivity and cell concentration in the reactor if Yx/O2 is 15 g cell/g oxygen assuming a feed flow rate of 50L/h