a to ion- *30-25. The following data are for a liquid chromato- graphic column 30- the On mL Length of packing Flow rate VM Vs 24.7 cm 0.313 mL/min 1.37 ml 0.164 mL ngs e a *30 of A chromatogram of a mixture of species A, B.C. and D provided the following data: ble. its of Width of Peak Base (W), min d 0.41 1.07 1.16 by Retention Time, min 31 (5.4 13.3 14.1 21.6 Nonretained A ne - D 1.72 of g- en re a Ec Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N.- (c) the plate height for the column. 30-26. From the data in Problem 30-25, calculate for A. B, C, and D (a) the retention factor. (b) the distribution constant. *30-27. From the data in Problem 30-25, calculate for species B and C (a) the resolution. (b) the selectivity factor. (c) the length of column necessary to separate the two species with a resolution of 1.5. (d) the time required to separate the two species on the column in part (c). 1 a