A two-stage chemostat system is utilised for secondary metabolite production. Growth occurs in the first reactor and the secondary metabolite is formed in the second reactor. The volumes of the first and second reactors are V = 0.6 m and V = 0.4 m respectively. The flowrate of the feed to the first reactor is 150 L/h. The concentration of the substrate in the feed is 6.0 g/L. Kinetic and yield parameters for the organism are: Hm = 0.35 h-1 Ks = 0.2 g/L Yxis =0.5 g/g qp =0.05 g P/g cell h YPIS = 0.75 g P/g Assume that product synthesis is negligible in the first reactor and growth is negligible in the second reactor. 1. Draw a diagram representing the system. (5) 2. Calculate the cell concentration and substrate concentration in the effluent of the first stage. (6) 3. Calculate the product concentration and substrate concentration in the effluent of the second reactor. (8)