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A volume of 10 L of a crude enzyme extract from a liver tissue was found to catalyze a reaction at a rate of 0.10
A volume of 10 L of a crude enzyme extract from a liver tissue was found to catalyze a reaction at a rate of 0.10 moles/min under optimal assay conditions. Following fractionation of 50 mL of the extract by ammonium sulfate precipitation, the resulting precipitate was re-dissolved in 10 mL of buffer. Using 10 L of the purified extract, the reaction rate was found to be 0.18 moles/min. Calculate the percentage recovery of the enzyme.
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