a) What is the molar extinction coefficient of this compound? b) A sample with the same compound but with an unknown concentration was analyzed with the spectrophotometer. Its absorbance was 0.82. What is the concentration of this compound? 5) A compound has an absorbency of 0.7. What will be its percent transmittance? 6) During the PCR lab we set the thermocycler with several cycles of temperature. Step 1: 91C for 2 min (Note Step 1 is done only once) Step 2: 94C for 30 sec Step 3: 50C for 30 sec Step 4: 72C for Imin 30 sec Step 5: Run 35 cycles from step 2 to 4 then after 35 cycles, go to Steps 6 and 7 Step 6: 72C for 10min Step 7. Hold at 4C a) What is the role of step 2, 3 and 4? b) Why was our first step at 94C longer? c) Why was step 6 at 72C longer? 7) When running a gel electrophoresis, should we place the DNA sample towards the positive or the negative end? Why? 8) What would happen if we chose the wrong primers to perform our PCR