a. What major mistakes did the researcher make in this preliminary analysis?

b. For this data, is a one way or two way ANOVA more appropriate?

c. Write a new Figure title for the reanalysed data taking into account the new conclusion (make sure it is descriptive)

d. Write a detailed Figure legend for this revised figure. Remember to refer to replicates, error bars, and statistical test chosen etc.

The data below shows the % apoptotic cells (dead cells) following treatment of the Hela cancer cell line with two different experimental drugs. One drug targets the PI3K signalling pathway and the other drug targets the MEK kinase signalling pathway. The vehicle is the control solution in which the experimental drugs (inhibitors) are dissolved in prior to their addition to the cancer cells. Table 1: Percentage of apoptotic Hela cancer cells following treatment with experimental drugs - PI3K and MEK kinase inhibitors. (Av = average; Jan = January). Av of n=3 Jan 10 3 Av of n=3 Jan 17 5 Av of n=3 Jan 24 2 Untreated cells 8 10 8 48 hr vehicle (control for the inhibitor) 48 hr P13K inhibitor (300NM GDC-0941) 48 hr MEK inhibitor (1uM U0126) 14 10 12 18 21 15 A researcher has analysed the data and concluded that both the PI3K and MEK inhibitor lead to a significant increase in cell death and has prepared Figure 2 to support their findings. They assumed normality due to low numbers of data points and unequal variances. P