a. You have tried to clone the following sequence that encodes for a novel protease

Please note that although the sequence is double-stranded, only one strand is shown for the sake of clarity. The convention is that you should be able to directly translate this to protein by using codon triplets.

Remind yourself what reading frames are. Only one of them is an open reading frame (start and stop in frame). Paste the sequence of the protein into your homework.

b. After cloning you want to confirm the sequence of your DNA. You design a primer with the following sequence:

TTAGAAGCGGTAACCAAC

If the sequence was cloned in correctly, write the first ten nucleotides you would obtain by sequencing?

ATGAAAAAAA TTGCATGTCT TTCAGCACTG GCCGCAGTTC TGGCTTTCAC CGCAGgTACT TCCGTAGCTG CGACTTCTAC TGTAACTGGC GGTTACGCAC AGAGCGACGC TCAGGGCCAA ATGAACAAAA TGGGCGGTTT CAACCTGAAA TACCGCTATG AAGAAGACAA CAGCCCGCTG GGTGTGATCG GTTCTTTCAC TTACACCGAG AAAAGCCGTA CTGCAAGCTC TGGTGACTAC AACAAAAACC AGTACTACGG CATCACTGCT GGTCCGGCTT ACCGCATTAA CGACTGGGCA AGCATCTACG GTGTAGTGGG TGTGGGTTAT GGTAAATTCC AGACCACTGA ATACCCGACC TACAAACACG ACACCAGCGA CTACGGTTTC TCCTACGGTG CGGGTCTGCA GTTCAACCCG ATGGAAAACG TTGCTCTGGA CTTCTCTTAC GAGCAGAGCC GTATTCGTAG CGTTGACGTA GGCACCTGGA TTGCCGGTGT TGGTTACCGC TTCTAA