A.Describe the results with WaTx in Fig. 2A, B and G and explain them methodically. - 2.5 pts.B.Describe the ion preference of TRPA1 under normal conditions and how this is affected by WaTx binding (Fig. 4F). - 2.5 pts.?C.Based on the article we discussed and a relevant literature search, diagram and describe with text the structure of TRPA1, its signaling mode, and the WaTx binding site in the context of its insertion into the plasma membrane. - 3 pts.

A B C Basal 0.37 5pA 5ms Basal - WaTx - AITC Basal WaTX AITC 1.0 WaTx * * * * Norm. count Avg. Cumul. Rel. Freq. w 0.5 200 N dwell Avg. LTFO 100 AITC O - 0.0- 0.0 5 10 15 20 200 400 600 Dwell Time (msec) Latency (msec) D E F 5pA 25ms Basal 0.005 Basal WaTX AITC IAM+IAM wash IAM+WaTx +IAM 400 * * * 10 * * * * 300 Y (PS) * * * * 200 IAM wash 100 * * * * Relative Permeability (Px/PNa) Norm. count Na Caz 5 IAM + WaTx dwell - N O WaTx wash 0 0 10 20 30 NMDG+ 0.04 Dwell Time (msec) Na CS K Ma2+ Cazx Figure 4. WaTx Is a Mode-Specific Gating Modifier (A-C) Inside-out HEK cell patch recordings (Vh = -40 mV) of spontaneous, WaTx (100 nM), or AITC (10 uM)-evoked TRPA1 openings (A), quantified with respect toA B Nat Basal WaTx WaTx + NMDG+ AITC AITC+Inhib. Na Basal WaTx WaTx + NMDG+ out AITC AITC+Inhib. Nat in +80--- 10pA|50ms Na man mean -Tow 10pA 50ms +80 +40- +40- -40 -40- -w -80 -- -80- - C 5A D E 5A 0.51 2_ 99 A 100 R = 1 180 0.4 0.3 50 Norm. RFU K (min-1) 0.2 10nM C 100nM 0. N 500nM WT N 1000nM K7A 0.0 500 1000 [Tx] nM WT IUPA | 5ms Na+ +80mV Na+ in Nat +80mV NL