After purifying tyrosinase, you wish to know how much total protein you have purified You use a Bradford protein assay to determine your protein concentration. Your sample containing tyrosinase has an absorbance reading of 0.655. You use BSA as your standard and receive the following data:

a. Draw your standard curve below. Don't forget to label your axes. 

b. What is the concentration of your sample if you used 25 μl? 

c. What is the specific activity of your enzyme if the relative activity is 45 U/μl?

BSA amount (ug) Absorbance at 595nm 0.5 1.0 2.0 4.0 0.205 0.410 0.805 1.600 1-8 1.4 1.21 0.8 D.u D.4 0.2 0.5 1.0 2.0 4.0 protein (g)