All the information is provided is in the picture the standard curve value, milk labels, their value, and and graph that is needed for question 5. This is all the information and references, nothing is missing.

1. Use Logger Pro to generate a standard curve for the BSA standards measured in the spectrophotometer. First plot concentration vs. absorbance. Then insert a trend line (linear regression line) to form the standard curve. Paste in a labeled copy of your graph as a figure here in your answer and make an appropriate descriptive legend below it (Figure 1). Description - see: Writing Papers in the Biological Sciences for good examples of legend writing. 2. Record the equation for the regression line. What does each value (y, m, x, b) in the equation represent? 3. What are the concentrations of the milk samples based on your calculations from the Bradford Assay? Use (and show working) of the regression line equation to calculate the concentrations of both samples. 4. Based on your calculations, does regular or ultrafiltered milk have more protein? Questions 4 and 5 Background Information: The Bradford assay has limits, it does not work for all protein concentrations. The assay is an indirect (or colorimetric) method for estimating protein concentrations. The Bradford assay is actually based on a shift in the absorbanceof the dye, Coomassie Brilliant Blue G-250,from 465 nm to 595 nm when it binds a protein under acidic conditions. That is, the Bradford assay is really measuring theamount of bound dye and NOT the amountof protein present (although the two areconnected until the dye is all used). As thedye is limiting (i.e., you only put in a setamount), then you can reach a point whenall the dye is bound, and no matter howmuch more protein you put in you will notsee a change in absorbance. That is, youcan increase the protein level to a pointwhere available dye becomes exhausted sothe graph would plateau. This concept is illustrated by the graphprovided in picture The milk samples used for lab werediluted 1:100. If they were not diluted,the dye would"used up" and theabsorbance would not be an accuraterepresentation of the proteinconcentration. 2. 5. The milk samples were diluted 1:100 inpreparation for your lab. This means that forevery 100ul of solution, there was 1ul of milkand 99 ul of water. When samples are dilutedprior to the Bradford assay, you need toexecuteanother calculation to compensate forthe dilution. What would be the proteinconcentrations of the undiluted samples? Hint:You need to executesomething with thenumber 100. 6. When examining a nutrition label, protein is.shown in amount per serving, which is 8oz(237ml). Calculate the mg of protein in 237mlof each of your samples to determine theamount of protein per serving. Hint: You needto executesomething with the number 237. 7. Protein is usually reported in grams onnutrition labels. Convert your numbers fromquestion 7 from mg to g. Hint: How many mgare in 1 g? 8. Look at the nutrition labels for the 2 milks.How does the protein amount shown on thelabels compare to your calculations? If yournumbers differ from the labels, provide at least1 reason why.

Regular milk: Nutrition Facts 8 servings per container Serving size 8 fl oz (240mL) Amount per serving Calories 160 Total Fat 9g Saturated Fat 5g % Daily Value Ultrafiltered milk: Nutrition Facts About 6 servings per container Serving size 1 cup (240 mL) Amount per serving Calories 120 Total Fat 4.5g Saturated Fat 3g Trans Fat 0g Cholesterol 20mg Sodium 120mg % Daily Value 6% 15% 7% 11% 5% 26% Total Carbohydrate 6g 2% Trans Fat 0g Dietary Fiber 0g 0% Total Sugars 6g Cholesterol 35mg 12% Includes Og Added Sugars 0% Sodium 105mg 5% Protein 13g 26% Total Carbohydrate 12g 4% Vitamin D 5mcg 25% Dietary Fiber 0g 0% Calcium 380mg 30% Potassium 400mg 8% Total Sugars 12g Vitamin A 120mcg 15% 0% Riboflavin 0.25mg 20% Vitamin B 1.1mcg 45% Protein B Phosphorus 245mg 20% Magnesium 2timg 6% Zinc 14mg 15% Selenium 10mcg 2016 Nora santicent sounds at inse me 14 Daly Vese vitesse cha Couried in a serving of fad.contributes B Table 1 for the stardand Protein Protein conc Table 11 for the milks Bradford Assay Standard Curve Protein concentration v. Absorbance ABS value Protein Protein conc ABS 0.00 2 0.01 mg/ml 0-21 3 0.25 mg/ml 0.70 Milk 1 (fl) 0.895 mg/ml Milk? (SB) 0.17 ng/ml 0.25 0.95 Maximum Absorbance Graph plateaus due to all the dye being bound by protein . 0.50 mg/ml 0.42 S 0.75 mg/ml. 0.98 b 1.00 mg/ml 1.03 so mg/ml 0.78 8 2.00 mg/ml 2:50 The milk samples used for lab were diluted 1:100. If they were not diluted, the dye would be "used up" and the absorbance would not be an accurate representation of the protein concentration. 1