Answered step by step
Verified Expert Solution
Question
00
1 Approved Answer
Alpha-amylase 1 is an enzyme that in humans is encoded by the AMY1A gene. Gene was amplified with PCR, cut with BamHI and SalI restriction
Alpha-amylase 1 is an enzyme that in humans is encoded by the AMY1A gene. Gene was amplified with PCR, cut with BamHI and SalI restriction and clones into pBR327 and transform in E. coli. These clones are plated on medium containing IPTG and X-gal, and Ampicillin. Explain the result of screening with diagram of these transformed clonies i.e having recombinant and nonrecombinant vector pBR327.
Step by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access with AI-Powered Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started