5. (1.5 points) Which level of structure would be most significantly perturbed in the following situations? Answer each question with primary, secondary, tertiary, or quaternary. a. Change (mutation) of a single amino acid that prevents two protein chains from associating b. Hydrolysis of the protein with 6M HCI for 24 hours, breaking all peptide bonds c. Replacing all backbone amide hydrogens with methyl groups