An analysis of a purified protein was performed by measuring its absorbance at $265$ nm in aqueous solution. The method blank was found to have an absorbance of $0\mathrm{.}002$ A solution of the protein was prepared without knowing its concentration.

This solution has an absorbance @ $265$ nm of $0\mathrm{.}224.$

What is the concentration of this unknown solution? What is the molecular weight of this protein if $1\mathrm{.}00$ mL of this unknown solution has an osmotic pressure of $3\mathrm{.}79$ millitorr? DIMS?

mg$/$mL of protein Absorbance @ $254$ nm

$20\mathrm{.}042$

$80\mathrm{.}136$

$120\mathrm{.}199$

$150\mathrm{.}254$

$180\mathrm{.}301$