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An experimental approach is planned with the aim to express a recombinant protein in E. coli followed by a purification step using an anti-6X
An experimental approach is planned with the aim to express a recombinant protein in E. coli followed by a purification step using an anti-6X His-tag antibody. However, for the protein to be used as a therapeutic, the His6-tag needs to be removed. Briefly explain how this could be achieved and how this could be included in the cloning design.
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To achieve the removal of the His6tag from the recombinant protein expressed in E coli a cleavage site for a specific protease can be introduced betwe...
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Fundamentals of biochemistry Life at the Molecular Level
Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt
4th edition
470547847, 978-0470547847
