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An ultrafiltration system is operated to concentrate a protein solution containing a buffer. The average pressure drop across the membrane is 3 0 kPa, and
An ultrafiltration system is operated to concentrate a protein solution containing a buffer. The average pressure drop across the membrane is kPa, and the fluid viscosity is kPa With clean membranes, the transmembrane flux using buffer in the system is liters for this pressure drop. After min of operation with the protein solution, the transmembrane flux is liters At this point, the system is operated with the buffer only in the backflushing mode with negative transmembrane pressure until a constant transmembrane flux is reached. Then, the system is operated with the buffer in the normal mode positive transmembrane pressure and the steadystate transmembrane flux is liters at a pressure drop of kPa. It has been determined that the flux due to the osmotic pressure difference is negligible. Estimate the irreversible fouling resistance and resistance due to concentration polarization and a gel layer for the membrane after min of operation with the protein solution.
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