fil Answer the following questions before you leave Talk with the instructor to make sure your answers are correct. You will need to discuss ALL of these questions in your lab report. LB/amp Plate 1 E. coll +PGLO plasmid LB/amp/arabinose Plate 2 LB/amp Plate 3 E. col -DGLO -pul Jaamid plasmid grow ston bos episiquey gazdo LB Plate 4 VU prizor 1. Which plate should contain the most number of cells growing (assume that transformation is not 100% efficient)? Why?way as woled sidst erit al 2. Which plate should contain the least number of cells growing? Why? entah pages 3. What do the control plates 1, 3, and 4 tell you (what is the purpose for each of these plates)? Give an explanation for each plate. For example, do you expect when growth or no growth on each plate? If you observed the opposite of what you expect, what does this mean? 4. Which plate will have green cells under a UV light? Why? 69 (AMO) 5. Which plates will have cells that are NOT green under a UV light? Why? (AMC-) 6. The (-DNA) tube does not contain the pGlo DNA, so the cells will not be transformed with the plasmid. Why does it still have to go through the heat shock process the same way as the (+DNA) tube (be specific)? will be the basis for t 81 7. An organism's environment can determine its physical trait (phenotype). a) After transformation is successful, what two factors must be present in order for you to observe the green fluorescence of the transformed cells (other than the pGlo)? Explain what each factor is doing to cause green fluorescence.