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Aqueous two - phase extraction is used to purify a recombinant Beta - galactosidase fusion peptide produced in Bacteria. For optimum separation, 5 0 0

Aqueous two-phase extraction is used to purify a recombinant Beta-galactosidase fusion
peptide produced in Bacteria. For optimum separation, 500 kg of a mixture of 17.7% w/w
polyethylene glycol (PEG) and 19.7% w/w potassium phosphate salt in water is needed.
Left over from previous pilot-plant trials is 50 kg mixture of 20% w/w PEG in water, and
200 kg of a mixture of 20% w/w PEG of a and 25% w/w salt in water. Also on hand is 200
kg of an aqueous stock solution of 50% w/w PEG, 200 kg of an aqueous stock solution of
40% w/w salt, and an unlimited supply of extra water. If all of both leftover mixtures must
be used, how much of each stock solution and additional water is required?

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