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Aqueous two - phase extraction is used to purify a recombinant Beta - galactosidase fusion peptide produced in Bacteria. For optimum separation, 5 0 0
Aqueous twophase extraction is used to purify a recombinant Betagalactosidase fusion
peptide produced in Bacteria. For optimum separation, kg of a mixture of ww
polyethylene glycol PEG and ww potassium phosphate salt in water is needed.
Left over from previous pilotplant trials is kg mixture of ww PEG in water, and
kg of a mixture of ww PEG of a and ww salt in water. Also on hand is
kg of an aqueous stock solution of ww PEG, kg of an aqueous stock solution of
ww salt, and an unlimited supply of extra water. If all of both leftover mixtures must
be used, how much of each stock solution and additional water is required?
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