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(b) The deprotection of fluorenylmethoxycarbonyl (Fmoc) amino acids is subject to catalysis by general bases in aqueous solution. A set of data is provided in
(b) The deprotection of fluorenylmethoxycarbonyl (Fmoc) amino acids is subject to catalysis by general bases in aqueous solution. A set of data is provided in the table below for the rates of deprotection of Fmoc-lysine at three different pHs, with p- chlorophenoxide as the base component of the buffer. Use these data to determine values for the rate constants kb, koh- and kh20 (where B=p-chlorophenoxide). 103 x kobs / s -1 pH 10% [p-chlorophenoxide] /M 1.50 8.78 2.00 1.70 8.78 4.80 1.90 8.78 7.60 2.80 9.08 2.00 3.10 9.08 6.20 3.40 9.08 10.20 5.50 9.40 1.60 5.70 9.40 4.40 6.00 9.40 8.60 (12 marks)
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