Based on the paper:

Clemmens, John, et al. "Mechanisms of microtubule guiding on microfabricated kinesin-coated surfaces: Chemical and topographic surface patterns." Langmuir 19.26 (2003): 10967-10974.

Consider the following flow cell:

a. Knowing the dimensions of the components of the above flow cell, approximately what volume of liquid can be introduced in it?

b. Considering that we have a kinesin solution of concentration 2 g/mL. If we introduce this solution in the flow cell, what would the kinesin density on the surface be? In other words, what would the spacing between kinesin motors be?

c. Could the distance between the kinesin motors explain the gliding phenomenon described by Clemmens et al.? Maybe a bit more clearly phrased: How does the distance between the kinesin motors affect the gliding of the microtubules described by Clemmens et al.?

Remember that the emphasis should be put on the order of magnitude of your result and the sensible deductions that you will make.

Consider the following flow cell: Dimensions: Coverslip: 2cm2cm Slide: 5cm2.5cm Spacer thickness: 100m Spacer width: 0.5cm