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Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ, 5, 141 (1971)]. The

Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ, 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30C. I (min) 0 10 20 so 100 Cno, (mol/L) 0.02 0.01775 0.0158 0.0106 (a) Determine the Michaelis-Menten parameters Vmax and KM. (b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes? 0.005

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