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Beer-Lambert yasasna gre, A = . c. L, burada A, absorbans; , snme katsays; C, konsantrasyon ve l, yol uzunluu (1 cm) Bir proteinin molekler
Beer-Lambert yasasna gre, A = . c. L, burada A, absorbans; , snme katsays; C, konsantrasyon ve l, yol uzunluu (1 cm)
Bir proteinin molekler arl 60.000 Da'dr. 1,00 mg/ml'lik bir solsyonun A280 deeri 0,750'dir.
a) Snme katsaysn cm2 mg-1 cinsinden hesaplaynz.
b) Molar snme katsaysn hesaplayn.
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