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Below is a calibration curve for the concentration of curcumin in methanol, collected using UV-vis spectroscopy. ance Absorban 1.400 1.200 1.000 0.800 0.600 0.400
Below is a calibration curve for the concentration of curcumin in methanol, collected using UV-vis spectroscopy. ance Absorban 1.400 1.200 1.000 0.800 0.600 0.400 0.200 0.000 0.000 0.010 y = 30x 0.020 0.030 [Curcumin] (mm) 0.040 0.050 Figure 1. Absorbance at 422 nm plotted against concentration for a freshly made solution of curcumin in methanol in a 1 cm quartz cuvette The rate of degradation of curcumin was also monitored in the methanol solution over time by monitoring the absorbance at 422 nm, Table 1 below. (i) How long would it take for a 25 M solution of curcumin to degrade to 3 M? Table 1. Absorbance of a solution of curcumin in methanol at 422 nm over 4 days Time (days) Absorbance 0.75 0.5 0.33 0.24 0.17 0.12 0.08 0.062 0.055 0 0.5 1 1.5 2 2.5 3 3.5 4
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