Biochemistry I Lab Amino Acid Paper Chromatography Worksheet 1. Collect the data and calculate the Rf (Retardation factor) value for each known and unknown amino acid: ( 30 points) Distance travelled by solvent Title: 2. Please draw a basic structure of amino acid. (2 points) 3. What conclusions can yourid 3. What conclusions can you draw about the affinity of amino acids to the mobile and stationary phase (in this experiment) based on their polarity? Explain the reason for that pattern. (4 points) 4. Based on your data: ( 4 points) a. Identify the amino acid that has the highest affinity towards stationary phase. b. Identify the amino acid that has the highest affinity towards mobile phase. 5. Please place the following amino acid in increasing degree of polarity based on their separation during paper chromatography. ( 6 points) Glycine, Proline, Alanine, Histidine, Valine, Leucine 6. Identify the amino acid(s) present in the unknown samples. (Explain only one unknown sample-your own). Describe your reasoning for coming to the conclusion. ( 4 points). Please include Rf Values and other qualitative information in your conclusion. , Minillis Biochemistry I Lab Amino Acid Paper Chromatography Worksheet 1. Collect the data and calculate the Rf (Retardation factor) value for each known and unknown amino acid: ( 30 points) Distance travelled by solvent Title: 2. Please draw a basic structure of amino acid. (2 points) 3. What conclusions can yourid 3. What conclusions can you draw about the affinity of amino acids to the mobile and stationary phase (in this experiment) based on their polarity? Explain the reason for that pattern. (4 points) 4. Based on your data: ( 4 points) a. Identify the amino acid that has the highest affinity towards stationary phase. b. Identify the amino acid that has the highest affinity towards mobile phase. 5. Please place the following amino acid in increasing degree of polarity based on their separation during paper chromatography. ( 6 points) Glycine, Proline, Alanine, Histidine, Valine, Leucine 6. Identify the amino acid(s) present in the unknown samples. (Explain only one unknown sample-your own). Describe your reasoning for coming to the conclusion. ( 4 points). Please include Rf Values and other qualitative information in your conclusion. , Minillis