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Biochemists use osmosis to calculate the molecular mass (MW) of an unknown protein. First, they dissolve exactly 1.00 mg in 1.00 ml of solution. At
Biochemists use osmosis to calculate the molecular mass (MW) of an unknown protein. First, they dissolve exactly 1.00 mg in 1.00 ml of solution. At this point they do not know the molarity of the solution because they do not know the MW of the protein. Then, they put this solution on one side of a semipermeable membrane and measure its osmotic pressure (1.35 torr) and experimentally find the molarity, and hence the MW of the protein. How much the molecular mass of an unknown protein? Note: The experiment is done at 298 K.
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