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Calculate how to make the following solutions and record in your lab notebook. Be sure to include the volume of solvent needed when appropriate. Solutions:
Calculate how to make the following solutions and record in your lab notebook. Be sure to include the volume of solvent needed when appropriate. Solutions: 1. 1ml TE buffer (10mM Tris, 1mM EDTA using stocks of 0.5M EDTA and 1M Tris) 2. 1ml50mM EDTA from stock of 0.5M EDTA 3. 5ml70% ethanol in water, from a stock of 95% ethanol 4. 1L of 1xTAE in water, from 50x stock solution 5. 80mL of a 1% agarose gel in 1xTAE 6. 200ml 0.5M EDTA (mw: 372.24) 7. 100ml 1M Tris (mw: 121.10) 8. 100ml 1M MgCl2 (mw: 95.21) 9. 100ml10% SDS 10. 10ml10mg/ml lysozyme 11. 15ml3M sodium acetate ( mw:136.08 )
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