Calculate the sample volume needed for the following dilution: 1 to 10 dilution, 90 L diluent.

2. Calculate the diluent volume needed for the following dilution: 1 to 5 dilution, 50 L total, 10 L sample.

3. Calculate the dilution made if 0.5 mL of sample was added to 9.5 mL diluent.

4. Calculate the dilution made if a 25 mL sample is added to a 175 mL diluent.

5. Calculate the sample volume if a 1 to 10 dilution was made with a total volume of 200 L.

6. Calculate the diluent volume for a 1 to 30 dilution if the sample volume is 10 L.

7. Calculate the dilution factor if the sample volume is 30 L and the diluent volume is 270 L.

8. Calculate the dilution factor if a 0.25 mL sample is added to 2.75 mL diluent.

9. Calculate the true analyte value given the following information: Analyte diluted using 10 L into 90 L diluent; Diluted analyte value = 85 mg/dL.

10. A 1 to 4 dilution is a 1 to ___ ratio.

11. If 10 L sample is added to 90 L diluent, what is the ratio?

12. A four-tube, 1 to 10 serial dilution was performed. What is the dilution factor of the second tube?

13. Given a series of 5 dilutions were performed. What is the final dilution?

14. Given a series of 4dilutions, what is the concentration in tube No. 3 if the original concentration was 100 mg/dL?