Calculate the standard error of regression (SER) for the Lineweaver-Burk slope and the Eadie-Hofstee slope to compare theprecision of each.

There are several ways to transform the Michaelis-Menten equation so as to plot data and derive kinetic parameters, each with different advantages depending on the data set being analyzed. One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver-Burk equation by Vmax and rearranging gives the Eadie-Hofstee equation. Vo = Vo (Km) + Vmax [S] Consider the plot of Vo versus for an enzyme-catalyzed Vo [S] reaction. The control reactions (blue curve) did not contain any inhibitor. V V max A B Slope = -K V [S] max K m