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Calculating colipase activity How to Calculate Colipase activity using titration method pH 8 Titrent Concentrstion 0 . 0 2 N NaOH Change Rate ( ml

Calculating colipase activity How to Calculate Colipase activity using titration method pH8
Titrent Concentrstion 0.02N NaOH
Change Rate (ml/min)0.085
Stock soluton activity 14000U/ml
I made working stoock of 1400u/ml
Amount of sample used 75uL
Total volume of Assay (Buffer +substrate 15ml
Calculate activity I used the following =(Rate (ml/min)* Titrant Concnetration )*Df
volume of enzyme 75ul
When I calculate antivity is only ~3000U/ml am I calculating this correctly please show your steps

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