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Calculation: In the first step of the experiment, you pipet 50 l of 4 mM CaCl into 7 ml final volume of a reaction cocktail.

Calculation: In the first step of the experiment, you pipet 50 l of 4 mM CaCl into 7 ml final volume of a reaction cocktail. Then, you then add 300 l of the cocktail to each reaction tube; with the other reagents, the reaction tube contains a total volume of 500 l. What is the concentration of the CaCl in the reaction tube?

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