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Can someone explain what determines the expression level in the given promoter region? I am really confused,, I know there are one positive element (-40
Can someone explain what determines the expression level in the given promoter region? I am really confused,,
I know there are one positive element (-40 to -20) and one negative element (-10 to +1) left in the promoter region (-60 to +1). And negative element (-70 to -60) gone.
To identify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5' deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region? Promoter Region Mutant number -100 Reporter gene expression +1 5 -80 -60 -40 -20 1 5 2 10 3 5 4 0 5 5 To identify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5' deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region? Promoter Region Mutant number -100 Reporter gene expression +1 5 -80 -60 -40 -20 1 5 2 10 3 5 4 0 5 5Step by Step Solution
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