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Can someone please answer both parts step by step.. Thanks! 1. A protein solution is centrifuged for a period of time, what will the concentration

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Can someone please answer both parts step by step.. Thanks!

1. A protein solution is centrifuged for a period of time, what will the concentration profile (C vs. x) in the centrifuge tube most closely resemble and why? (a) (b) C top X bottom top X bottom (c) (d) top bottom bottom 2. If we carry out a density gradient centrifugation experiment in a CsCl solution, and the density gradient is as shown below, at what location in the centrifuge tube would a protein of density 1.4 g mL end up when the experiment reaches equilibrium and why? How does this method work 1.8 1.6 - 5) 0 1.4 1.2- 1.0- 30 40 32 34 36 38 x (cm)

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