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can you please answer all the parts please step by step please 7. Determination of Michaclis-Menten Kinetics (30 points): Consider the following enzyme-catalyzed reaction of
can you please answer all the parts please step by step please
7. Determination of Michaclis-Menten Kinetics (30 points): Consider the following enzyme-catalyzed reaction of urea: The rate of reaction is given below as a function of the urea (substrate S ) concentration a) Using Excel, determine the Michaelis-Menten rate equation parameters rmax( also called Vmax ) and Km by using the Lineweaver-Burk, Eadie-Hofstee and Hanes-Woolf equations (three separate plots). Show the model-predicted fit and clearly identify the three sets of rate equation parameters along with the regression statistics. (15 points). b) Using the above three sets of rate equation parameters, generate predicted values for ( rurra) vs CS from Cs=0 to Cs=0.90 using a spacing of Cs=0.01. Compare the original rurca vs Cs data (solid points) with the predicted values using the rate equations parameters identified in step 2. (LineweaveBurk - solid line; Eadie-Hofstee-dashed line, and Hanes-Woolf-dotted line). Comment on which of the three linear regression approaches performed the best in predicting the original reaction rates. ( 15 points) Step by Step Solution
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