CAN YOU TRACE ANY KIND OF CONNECTIVITY IN THESE QUETSIONS

1. The breaking of bonds,which always absorbs energy and increases mass.
2. The formation of bonds, which always releases energy and decreases mass.
3. The transformation of existing bonds which is really the excitation of the system to different states (always absorbs energy and increases mass) and de-excitation of the system to different states (always releases energy and decreases mass).
4. The creation of particles (always absorbs energy and increases mass) and annihilation of particles (always releases energy and decreases mass).

Note that when a chemical reaction absorbs energy, and therefore gains mass, it's not like electrons are created. The extra mass is not caused by the appearance of new particles. Rather, the extra mass is held in the system as a whole. Depending on the position and state of particles in a system relative to each other, the system gains or loses mass in addition to the individual masses of the particles. This concept is very similar to the classical concept of potential energy, but we now know that the energy is actually stored as mass. If you measure with very sensitive equipment the sum of the mass of two million hydrogen atoms and one million oxygen atoms that are separated from each other, then measure the mass of one million water molecules, you will find theses masses to be different.

QUESTIONS

1. state on the characteristics of the purity of enzymes

2.explain how Separates proteins of identical molecular weight that differ in pI hold for the dimensional electrophoresis?

3.how is SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge attributable to SDS polyacrylamide chromatography?

4.provide the sets of information that contradict the reality of ion-exchange chromatography

5.what justification holds for the true about affinity chromatography?

6.state the charecteristics ofsize-exclusion chromatography?

7.elabortae on the relationship betyween column chromatography and resolution increases subject to length of the column

8. The pattern on paper in chromatography is called __________explain briefly

9.in what form is Mobile phase ?

10.explain on the events of salting out effect during extraction of proteins