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Cibracon blue F 3 G - A is a competitive inhibitor of some enzymes. You were asked to determine whether Cibracon blue F 3 G
Cibracon blue FGA is a competitive inhibitor of some enzymes. You were asked to determine whether Cibracon blue FGA covalently attached to agarose would be useful for the chromatographic separation of aldehyde dehydrogenase. The inhibition constant Kin for inhibition of aldehyde dehydrogenase by Cibracon Blue FGA is mu M the inhibition constant is the dissociation constant for the enzymeinhibitor complex Assuming this same value applies for the reaction between the enzyme and the immobilized dye, estimate the velocity of the enzyme through a column packed with Cibracon Blue FGA immobilized to agarose epsi Assume that the effective dye concentration is mu mols Cibracon Blue FGA per milliliter bed volume, that the enzyme is applied at a concentration of mM and travels through the column in plug flow shock wave and that the solvent velocity through the column is cmsec State any other assumptions you must make to complete your analysis.
The answer was given as cmsec How do I get this answer
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