Circle the residues that would produce a peak in a fluorescence spectrum: REYPWNGHF

5QFGF PH 10(EM), 5Q FGF pH 7EM 1000- 0 nm 300 350 400 450 The fluorescence spectrum of folded FGF-1 shows a peak near 305 nm due to the fluorescence of multiple Tyr residues The fluorescence of the single Trp is not observed due to the presence of nearby pyrrole and imidazole groups Denaturation can be monitored by the shift of the emission maximum from 305 nm to 350 nm due to the exposure of the indole ring of tryptophan to the solvent bulk. Black = pH 10 (unfolded) Blue = pH 7 (folded) Red = pH 2 (unfolded)