Cleavage of ATP to AMP and PP; during Metabolism Synthesis of the activated form of acetate (acetyl-CoA) is carried out in an ATP-dependent process: Acetate + CoA + ATP acetyl-CoA + AMP + PP (a) The AG" for the hydrolysis of acetyl-CoA to acetate and CoA is -32.2 kJ/mol and that for hy- drolysis of ATP to AMP and PP; is -30.5 kJ/mol. Calculate AG" for the ATP-dependent synthesis of acetyl-CoA. (b) Almost all cells contain the enzyme inorganic pyrophosphatase, which catalyzes the hydrolysis of PP, to P. What effect does the presence of this enzyme have on the synthesis of acetyl-CoA? Explain.